Figure 5. Lipid mixing assay for the truncated SNARE complex.
Fluorescence changes normalized against the maximum fluorescence intensity (MFI) are shown for the lipid: protein molar ratio of 100:1 (A) and 200:1 (B). Wild-type SNAP-25 and the truncated SNAP-25A (Δ9) and SNAP-25E (Δ26) are used in the proteoliposome fusion assay. The control runs with the v-SNARE vesicles and the t-SNARE vesicles without the SNAP-25 (black curve).
