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. 2015 Jun 30;3:e1065. doi: 10.7717/peerj.1065

Figure 6. EPR spectra of the spin-labeled full-length syntaxin 1A mutants involved in the wild-type or truncated SNAP-25 and soluble VAMP2 ternary complex.

Figure 6

(A) and (B) EPR spectra for spin-labeled positions at 199, 206, 248 and 249 of syntaxin 1A in the ternary SNARE complex. These four positions correspond to the upstream of SNAP-25 SN2 neurotoxins cleavage sites. (A) is the ternary complex in the detergent, (B) is in the membrane. (C) and (D) EPR spectra for positions at 256, 257, 258 and 259 of syntaxin 1A in the ternary SNARE complex. These four positions are the last four amino acids of the syntaxin 1A SNARE motif. (C) is the ternary complex in the detergent, (D) is in the membrane. The mobile and immobile components are indicated in red and blue arrows, respectively.