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. 2015 Jul 7;5:11883. doi: 10.1038/srep11883

Figure 2. Detection of LptD and LPS intermediates at the N-terminal domain and the hydrophobic intramembrane hole.

Figure 2

The positions of residues where cross-linking with LPS was detected are shown in magenta; residue positions with no LPS cross-linking are shown in yellow. a residues for incorporating pBPA at the N-terminal domain. b residues are selected for the incorporation of pBPA at the intramembrane hole. c the top lanes are western blots for detecting LptD and LPS complexes at the N-terminal domain, while the bottom lanes are the protein expression levels of the LptD variants. LptD/LPS complexes were observed at residues V52, V104, A126, L128 and Y112. d the top lanes are the detection of the LptD and LPS complexes at the intramembrane hole, and the bottom lanes are the protein expression levels of the LptD variants. The protein and LPS complexes are captured at residues A196, I198, F203, L218, V220 and F228.