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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Nature. 2004 Oct 21;431(7011):1011–1017. doi: 10.1038/nature02964

Figure 2.

Figure 2

CDK1 is required for homologous recombination. a, MAT switching is initiated by creating an HO-induced DSB at the MAT locus that is repaired by gene conversion from HML or HMR. MAT switching is shown in asynchronous cells or cells arrested in G1 (α-factor arrest) or G2/M (nocodazole arrest). b–d, MAT switching in cdc28-as1 (b), wild-type (c) and cdc7-as3 (d) strains with and without 1-NMPP1 inhibitor. e, Allelic recombination in a cdc28-as1 homozygous diploid strain. The position of two DNA probes is shown: MAT-distal (MD, verifies DSB induction) and MAT-proximal (MP, detects product). f, Efficiency of NHEJ in cdc28-as1 cells with either active or inactive CDK1 at different stages of the cell cycle. Error bars indicate s.d.