Table 2.
Percentage contribution of different cell wall features of Miscanthus sinensis to saccharification (A and C) and recalcitrance (B and D) based on the results from principal component analysis
(A, B) Considering the three genotypes, showing the contribution for saccharification and recalcitrance without the interference of lignin content in PC1, (C, D) considering the results from PCA between H0120 and H0116, where lignin content interferes in saccharification in PC1.
| Without lignin interference | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| (A) Positively correlated to saccharification (favouring saccharification) | (B) Negatively correlated to saccharification (favouring recalcitrance) | ||||||||||
| Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | ||
| Pectins | RG1-b | Sodium chlorite | 2.05 | 27.9% | Xyloglucan | NFXG-3 | Ammonium oxalate | 2.86 | 25.9% | ||
| Linseed-MUC | 2.03 | NFXG-4 | 2.79 | ||||||||
| RG-1 | 2.00 | NFXG-2 | 2.74 | ||||||||
| AG-1 | 1.89 | NFXG-1 | 2.66 | ||||||||
| HG-2 | 0.1M NaOH | 2.08 | NFXG-5 | 2.64 | |||||||
| RG1-b | 2.08 | FXG | 2.58 | ||||||||
| Linseed-MUC | 2.06 | NFXG-2 | 4M NaOH | 2.48 | |||||||
| AG-1 | 2.06 | NFXG-1 | 2.41 | ||||||||
| AG-3 | 2.05 | FXG | 2.43 | ||||||||
| AG-2 | 1.95 | NFXG-3 | 2.33 | ||||||||
| RG-1 | 1.90 | Xylan | Xylan-3 | Ammonium oxalate | 2.94 | 12.9% | |||||
| RG-1 | 1M NaOH | 2.00 | Xylan-2 | 2.30 | |||||||
| RG1-b | 1.90 | Xylan-1 | 2.46 | ||||||||
| AG-3 | 1.89 | Xylan-2 | 4M NaOH | 2.61 | |||||||
| Mannans | GM-2 | Ammonium oxalate | 2.00 | 16.0% | Xylan-3 | 2.56 | |||||
| ACMAN | 2.01 | Pectins | RG1-a | Ammonium oxalate | 2.76 | 7.9% | |||||
| GM-2 | Sodium chlorite | 2.03 | HG-1 | 2.56 | |||||||
| ACMAN | 2.03 | RG1-a | Residue | 2.56 | |||||||
| GM-2 | 0.1M NaOH | 2.08 | Galactose (%) | 4M NaOH | 2.84 | 2.8% | |||||
| ACMAN | 2.10 | % Hemicellulose | 2.48 | 2.5% | |||||||
| GM-2 | 1M NaOH | 1.87 | |||||||||
| ACMAN | 1.87 | ||||||||||
| β-Glucan | BG | 0.1M NaOH | 2.01 | 3.9% | |||||||
| BG | 1M NaOH | 1.87 | |||||||||
| Xylan-5 | Xylan-5 | Sodium chlorite | 2.05 | 2.0% | |||||||
| With lignin interference | |||||||||||
| (C) Positively correlated to saccharification (favouring saccharification) | (D) Negatively correlated to saccharification (favouring recalcitrance) | ||||||||||
| Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | ||
| Xylan | Xylan-4 | Ammonium oxalate | 1.84 | 19.8% | Pectins | AG-1 | Sodium chlorite | 3.31 | 28.2% | ||
| Xylan-7 | 1.79 | AG-2 | 3.75 | ||||||||
| Xylan-5 | 0.1M NaOH | 2.19 | AG-3 | 3.56 | |||||||
| With lignin interference | |||||||||||
| (C) Positively correlated to saccharification (favouring saccharification) | (D) Negatively correlated to saccharification (favouring recalcitrance) | ||||||||||
| Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | Polysaccharide | Variable | Extract | % from PC1 | Total % per polysaccharide | ||
| Xylan-6 | 2.16 | AG-4 | 3.87 | ||||||||
| Xylan-7 | 1.91 | RG/AG | 3.75 | ||||||||
| Xylan-1 | 1M NaOH | 1.95 | Linseed-MUC | 3.45 | |||||||
| Xylan-4 | 2.16 | AG-1 | 4M NaOH | 3.22 | |||||||
| Xylan-5 | 2.10 | HG-2 | Residue | 3.25 | |||||||
| Xylan-6 | 2.06 | Xylan | Xylan-1 | Ammonium oxalate | 2.89 | 9.6% | |||||
| Xylan-1 | 4M NaOH | 1.7 | Xylan-1 | Sodium chlorite | 3.05 | ||||||
| Xyloglucan | FXG | Ammonium oxalate | 1.94 | 16.9% | Xylan-2 | 4M NaOH | 2.89 | ||||
| NFXG-2 | 1.94 | β-Glucan | BG | 1M NaOH | 3.39 | 6.7% | |||||
| FXG | 1M NaOH | 1.78 | BG | Residue | 3.33 | ||||||
| NFXG-1 | 2.08 | Xylose (%) | Ammonium oxalate | 3.33 | 3.3% | ||||||
| NFXG-2 | 1.83 | Lignin (%) | 2.97 | 3.0% | |||||||
| NFXG-4 | 1.81 | ||||||||||
| NFXG-5 | 1.87 | ||||||||||
| NFXG-6 | 1.95 | ||||||||||
| NFXG-6 | 4M NaOH | 1.73 | |||||||||
| Pectins | Linseed-MUC | 0.1M NaOH | 1.76 | 9.3% | |||||||
| RG1-b | 1M NaOH | 2.06 | |||||||||
| HG-1 | 1.94 | ||||||||||
| Linseed-MUC | 1.83 | ||||||||||
| RG/AG | 1.73 | ||||||||||
| Glucose (%) | Ammonium oxalate | 2.02 | 2.0% | ||||||||