Table 3.
Weight percent amounts of sugars released from cell wall polysaccharides, and relative cell wall crystallinity, comparing BdPAL RNAi knocked-down stem tissue with the respective control
WT and RNAi1-1 plants were grown together and should be compared with each other, as were CTL and RNAi1-3.
| Sample | Glucose | Xylose | Arabinose | Galactose | Mannose | Rhamnose | Fucose | Crystallinity index |
|---|---|---|---|---|---|---|---|---|
| WT | 42.4±1.0 | 25.0±0.5 | 2.99±0.07 | 0.68±0.03 | 0.12±0.01 | 0.074±0.007 | 0.034±0.002 | 0.341±0.002 |
| RNAi1-1 | 44.3±0.8 | 26.1±.0.3 | 3.97±0.11 | 0.82±0.02 | 0.14±0.01 | 0.090±0.009 | 0.038±0.003 | 0.360±0.011 |
| P-value | 0.06 | 0.03 | 0.0002 | 0.002 | 0.18 | 0.09 | 0.12 | 0.04 |
| CTL | 43.7±0.9 | 24.9±.0.7 | 2.83±0.06 | 0.70±0.03 | 0.13±0.004 | 0.085±0.008 | 0.033±0.002 | 0.324±0.002 |
| RNAi1-3 | 46.4±0.7 | 26.1±.0.4 | 3.29±0.09 | 0.76±0.02 | 0.14±0.002 | 0.078±0.021 | 0.030±0.002 | 0.340±0.012 |
| P-value | 0.02 | 0.1 | 0.002 | 0.03 | 0.007 | 0.6 | 0.23 | 0.09 |
Values are means ±SD. P-values comparing the knock-down line with its control were calculated using the Student’s t-test. n=3 independent pools of senesced stem plus leaf sheath tissues (three technical reps for each).