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. 2015 Jun 19;66(14):4239–4250. doi: 10.1093/jxb/erv283

Fig. 2.

Fig. 2.

qPCR-RBIP. (A) Flow chart of the method used. (B) Schematic of the two qPCRs. Black arrows show the location of the primers, and triangles are the LTRs of the scIvana elements, depicted in grey. Flanking regions are shown as a dark grey line. Fluorescent probes used for qPCR are shown as curved arrows. When the scIvana element is present at the locus, forward primer 2 and the reverse primer are able to amplify the occupied site, while forward primer 1 and the reverse primer will not amplify because the resulting product would be too long to amplify under the PCR conditions chosen. When the scIvana element is absent at the locus, forward primer 1 and the reverse primer are able to amplify the non-occupied site because the ~5kb scIvana is not present. (This figure is available in colour at JXB online.)