Figure 2.

Effects of the specific surface area of silica particles on binding to human plasma proteins. Each protein solution was mixed with SP70, nSP100, nSP300, or mSP1000 at 1.75, 2.5, 7.41, and 25 mg/mL, respectively (i.e., approximately 1.5 × 10⁴ mm² of specific surface area). After centrifugation, each sample was separated by SDS-PAGE. The gel was stained by CBB staining, and the protein bands of albumin (A), transferrin (B), IgG (C), and fibrinogen (D) were quantified with the ImageJ software. The protein content of each band was estimated from its optical density compared with the optical density of each standard solution. Data are presented as the mean ± SD; n = 3; **P < 0.01 vs. nSP70-treated group; *P < 0.05 vs. nSP70-treated group; N.D., not detected.