Table 1.

Oligonucleotide primers (specific to mcrB, mcrG, mtaB, and mtbA genes) and PCR conditions.

Gene	Name and sequence of the oligonucleotide primer*	PCR product size	PCR conditions**
mcrB	LMCRB: 5′- TWYCARGGHYTVAAYGC -3′ RMCRB: 5′- CCDCCDCCDCCRTARAT -3′	~392 bp	96°C–30 s; 56°C–30 s; 72°C–40 s 39 cycles
mcrG	LMCRG1: 5′-CAYCCDCCDYTNGADGARATGGA-3′ RMCRG1: 5′-TCRAACATYANWCCRTYYTCRTC-3′	~356 bp	96°C–30 s; 56°C–30 s; 72°C–35 s 39 cycles
mtaB	LMTAB: 5′- CARGCHAAYACYGCMATGTT -3′ RMTAB: 5′- CYTGDGGRTCYCKGTA -3′	~436 bp	96°C–30 s; 56°C–30 s; 72°C–40 s 39 cycles
mtbA	LMTBA: 5′- TTCTCCCTTGCMCAGCA -3′ RMTBA: 5′- ACWGGRTCVAGRTTWCC -3′	~413 bp	96°C–30 s; 55°C–30 s; 72°C–40 s 39 cycles

^*IUPAC code: A (adenine), C (cytosine), G (guanine), T (thymine), R (A or G), Y (C or T), W (A or T), K (G or T), M (A or C), D (A or G or T), H (A or C or T), V (A or C or G), N (A or C or G or T).

^**PCR conditions were specified for Taq DNA polymerase (Qiagen). The applicability of other (high fidelity) polymerases [i.e., Phusion High-Fidelity DNA Polymerase (Thermo Scientific) and KAPA polymerase (Kapa Biosystems)] was also confirmed.