Table 2.

Summary of bioinformatic analysis of sequenced mcrA, mcrB, mcrG, mtaB, and mtbA amplicons.

Sample*	Number of paired reads	Number of merged reads	Number of inferred peptides**	Primer sensitivity (% of correct product)
mcrA_AD	17,365	12,816	11,931	93
mcrA_WD	9277	4318	2572	59
mcrB_AD	32,094	23,188	21.939	94
mcrB_WD	50,485	40,242	25,035	57
mcrG_AD	42,185	29,330	21,988	74
mcrG_WD	34,945	28,660	18,272	63
mtaB_AD	26,500	20,753	19,163	92
mtaB_WD	36,148	15,293	13,231	86
mtbA_AD	33,770	22,852	10,027	43
mtbA_WD	31,601	19,150	10,961	57

^*AD, agricultural biogas plant anaerobic digester; WD, wastewater treatment plant anaerobic digester.

^**Inferred peptides number denote how many peptides that are sufficiently long and similar to a target protein can be extracted from the reads. Percent of correct product is the ratio between number of peptides and number of reads.