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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: J Mol Biol. 2008 Mar 4;378(2):337–352. doi: 10.1016/j.jmb.2008.02.056

Fig. 1.

Fig. 1

Depletion of calcium from ER or inhibition of ER glucosidases rescues cell surface expression of GAT1-E101D mutant. (a) Fluorescence microscopy of HEK293 cells transfected with GAT1-Δ37 or GAT1-E101D and treated with 1 μM thapsigargin. Cell surface expression of GAT1-E101D is evident after 4 h. (b) Whole-cell [3H]GABA uptake by GAT1-Δ37 or GAT1-E101D (c) following treatment with 1 μM thapsigargin (thapsi) or 1 mM castanospermine (CAS). The data shown are means of two independent experiments; error bars indicate SD.