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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: J Mol Biol. 2008 Mar 4;378(2):337–352. doi: 10.1016/j.jmb.2008.02.056

Fig. 5.

Fig. 5

Calnexin retains its chaperone function within the concentric bodies. (a) The temperature-sensitive mutant of VSVG was co-expressed with TM-calnexin (TM-CNX); tsVSVG did not colocalize with TM-calnexin if cells were cultured at 37 °C (left). After 4 h at 40 °C, VSVG colocalized with TM-calnexin in all observed concentric bodies (middle). Four-hour pretreatment of cells with 1 mM castanospermine (CAS) reduced the number of concentric membrane bodies in which TM-calnexin and tsVSVG colocalized. (b) Quantification of tsVSVG in concentric bodies: the fraction thereof in which tsVSVG and TM-calnexin were colocalized is indicated by the filled area of the bar (tsVSVG, n=43; tsVSVG at 40 °C, n=128; tsVSVG at 40 °C+CAS, n=71). (c) Confocal microscopy reveals release of tsVSVG from multilamellar bodies and its clustering within, near or around the TM-calnexin-labelled regions of the multilamellar bodies. Arrowheads indicate the select multilamellar bodies and adjacent tsVSVG clusters.