Skip to main content
. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: J Mol Biol. 2008 Mar 4;378(2):337–352. doi: 10.1016/j.jmb.2008.02.056

Fig. 6.

Fig. 6

GAT1 and E101D colocalize with calnexin and TM-calnexin in concentric bodies. (a–d) HEK293 cells were co-transfected with plasmids driving the expression of CFP-tagged GAT1 (a, b) or of CFP-tagged GAT1-E101D (c, d) and of YFP-tagged calnexin or of YFP-tagged TM-calnexin, as indicated. Proteins were visualized by epifluorescence microscopy as outlined under Materials and Methods. The right-hand panels in each row represent FRETc images (computed as in Fig. 3 according to the equation in Materials and Methods); results for each pair of interacting proteins are summarized in Fig. 8.