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. 2015 Feb 21;9(6):1155–1168. doi: 10.1016/j.molonc.2015.02.007

Figure 4.

Figure 4

DSF acts as an ionophore to induce Ctr1‐independent Cu uptake. A, Cu content (ng) normalized to protein (mg) in SUM149 (blue bars) and rSUM149 (green bars) treated with DSF alone or in combination with Cu measured by ICP‐HRMS. *p < 0.05, **p < 0.005. B, Ctr1 expression in normal and IBC cell lines. ←g indicates glycosylated form, ←t indicates truncated form. GAPDH as loading control. C, Left: Viability of SUM149 cells treated with Ctr1‐targeting siRNAs (gray and purple bars) or control luciferase‐targeting siRNA (white bars) following DSF (300 nM), Cu (10 μM), or DSF‐Cu (100–300 nM, 10 μM) treatment for 24 h. Right: Immunoblot analysis of SUM149 cells treated with Ctr1‐targeting siRNAs (A and B) or control luciferase‐targeting siRNA. D, Growth of SEY6210 (blue bars) and Ctr1/3‐deficient MPY17 (red bars) S. cerevisiae cells in YPEG media with addition of ZPT (left) or DSF (right) measured by absorbance at 600 nm.