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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Free Radic Biol Med. 2014 Mar 6;71:402–414. doi: 10.1016/j.freeradbiomed.2014.02.023

Figure 2. Abrogation of Nrf2 down regulates the bio-signature of antioxidants upon AEES.

Figure 2

Real-time RT-PCR determinations of Nrf2 target genes in WT and Nrf2-null mice under sedentary and AEES conditions at >23 months were performed using Qiagen-mouse primer sets (n=4/gp.). Data were first normalized to Arbp1 expression and then to the corresponding gene expression in the WT group (Delta-delta-CT method). While there is an increasing trend in the messenger RNA (mRNA) expression for major targets of Nrf2 (i.e. catalase, G6pd, Nqo1 and Gst-mu) were significantly upregulated in WT upon AEES, most of these genes were down regulated in Nrf2-null mice under sedentary or AEES conditions. The data are from n=3-4 mice/group. (*p<0.05 vs. WT-SED).