A) Representative Immunoblotting (IB) experiments of cytosol from WT and Nrf2-null mice under sedentary and AEES conditions at >23 months were performed using appropriate antibodies (n=4/group.). Protein blots were probed with anti-Nqo1, catalase, glucose-6-phosphate dehydrogenase, super oxide dismutase-1/2 (Sod-1/2), glutathione reductase, glutathione peroxidase and GAPDH. Each lane indicates an individual mouse.
B) Densitometry analysis was performed as relative intensity values calculated as mean arbitrary units obtained from the IB shown in (A). Significant decreases in NQO1, CAT, G6PD, SOD1 and GPX1 in Nrf2-null when compared to WT were evident under sedentary state. AEES upregulates most of the antioxidant proteins, but this effect was moderate in Nrf2-null mice. (*p<0.05, **p< 0.01, vs. WT-SED).