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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Free Radic Biol Med. 2014 Mar 6;71:402–414. doi: 10.1016/j.freeradbiomed.2014.02.023

Figure 9. Sustained impairment of skeletal muscle regeneration in Nrf2-null mice after post-AEES recovery.

Figure 9

Effect of recovery from AEES on skeletal muscle regeneration. In the post-AEES recovery experiments, we determined transcript (Fig. 9A-B) and protein levels for PAX7 and MyoD1 (9C).

(A-B) RTPCR experiments showing downregulation of mRNA levels (PAX7 and MyoD1) were prolonged even after 1 week of recovery from AEES.

(C) Representative Immunoblots for PAX7 and MyoD1 protein levels in WT mice showed a trend of normalization in response to recovery from AEES (compare lane 1 vs 3; Fig. 9C). Notably, down regulation of these proteins were sustained in Nrf2-null mice versus WT (compare lane 3 vs lane 4; Fig. 9C) following recovery from AEES. (n=4/group; *p<0.05).