Table 2.
Primer sequences for real-time PCR amplification
| Target genes | Reference/GenBank accession | Primer sequences |
|---|---|---|
| TLR2 | NM_213761.1 | F: GACACCGCCATCCTCATTCT R: CTTCCCGCTGCGTCTCAT |
| TLR4 | AB188301.2 | F: TCTACATCAAGTGCCCCTAC R: TAAATTCTCCCAAAACCAAC |
| NF-κB p65 | KC316023.1 | F: GGGGACTACGACCTGAATGC R: CACGGTTGTCAAAGATGGG |
| TNF-α | X57321.1 | F: CCACGCTCTTCTGCCTACTGC R: TCGGCTTTGACATTGGCTACAA |
| IL-1α | NM_214029.1 | F: TACTGACTATGGCTACCAA R: ATTCCAGCTGCTATTGTG |
| IL-1β | AY291592.1 | F: CCGCCAAGATATAACTGAC R: GCAGCAACCATGTACCAA |
| IL-6 | AF518322.1 | F: AATGCTCTTCACCTCTCC R: CACACTTCTCATACTTCTCAC |
| ACACA | NM_001114269.1 | F: GGCCATCAAGGACTTCAACC R: ACGATGTAAGCGCCGAACTT |
| FASN | NM_001099930.1 | F: GTCCTGCTGAAGCCTAACTC R: TCCTTGGAACCGTCTGTG |
| SCD | NM_213781.1 | F: CTACACAACCACCACTACCATCAC R: GCAAACGCCCAGAGCAAGG |
| HSL | HM591297.1 | F: ACCCTCGGCTGTCAACTTCTT R: TCCTCCTTGGTGCTAATCTCGT |
| ATGL | EU373817.1 | F: ACCTGTCCAACCTGCTGC R: GCCTGTCTGCTCCTTTATCCA |
| CPT-1A | NM_001129805.1 | F: ACAACGAGGTCTTCCGAT R: AACGCAAAACCACCAAACCC |
| UCP2 | NM_214289.1 | F: GACGCCTACAAGACCATC R: CTCAGCACAGTTGACAATG |
| UCP3 | NM_214049.1 | F: GACGATGGATGCCTACAG R: CACCTTCTCCTTGATGACA |
| 11β-HSD1 | NM_214248.1 | F: CCATGCTGAAGCAGAGCAAC R: AAGAACCCGTCCAGAGCAAA |
| ZAG | XM_003124307.2 | F: GCAGCCGTGAACACCAAGC R: GGTACCTCCGCAGCATCCC |
| adiponectin | EF601160.1 | F: CCGTTCAGCATTCAGTGT R: CAGCCTTGTCCTTCTTGTA |
| leptin | NM_213840.1 | F: CATCCATTGTTCGCTGTG R: CTGTCCTCTCCATTAGTCTC |
| PPIA | JX523418.1 | F:GACTGAGTGGTTGGATGG R:TGATCTTCTTGCTGGTCTT |
TLR2 toll-like receptor 2, NF-κB p65 nuclear factor-kappa B p65, TNF-α tumor necrosis factor-α, IL-1α interleukin-1α, ACACA acetyl-CoA carboxylase alpha, FASN fatty acid synthase, SCD stearoyl-CoA desaturase, HSL hormone-sensitive lipase, ATGL adipose triglyceride lipase, CPT-1A carnitine palmitoyltransferase 1A, UCP2 uncoupling protein 2, 11β-HSD1 hydroxysteroid (11-beta) dehydrogenase 1, ZAG zinc-α2-glycoprotein, PPIA Peptidylprolyl isomerase A