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. 2015 Jul 6;210(1):947–956. doi: 10.1083/jcb.201501013

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© 2015 Schouteden et al.

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Figure 2. — Ciliogenesis is largely normal in transition zone mutants. (A) Phasmid cilia in wild-type and transition zone mutants visualized by the IFT marker CHE-11:GFP (arrowheads). (B and C) Quantitation of phasmid cilia number per animal (B, n > 50 animals) and length (C, n > 25 cilia) based on CHE-11:GFP. Error bars indicate the 95% confidence intervals. Asterisks indicate statistically significant difference to wild-type (t test, P < 0.01). (D) Stills and kymographs from time-lapse sequences of IFT in phasmids of wild-type and transition zone triple mutants expressing CHE-11:GFP. (E) Anterograde IFT rates in the middle segment of wild-type and transition zone mutant phasmids (n > 40 particles per strain). Error bars indicate the 95% confidence interval. (F) Transmission electron micrographs of amphid cilia at the level of the middle segment of the axoneme in wild-type, ccep-290Δ, ccep-290Δ;nphp-4, and ccep-290;mksr-2;nphp-4 mutants. Axonemes were essentially normal except for occasional displaced doublet microtubules (arrowheads). Doublet microtubule number was not significantly affected (8.7 ± 1.3 2×MTs/cilium, n = 38 wild type; 7.7 ± 1.8 2×MTs/cilium, n = 27 triple mutant; t test, NS). Bars: (A and D) 5 µm; (F) 200 nm.