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. 2015 Jul 6;210(1):63–77. doi: 10.1083/jcb.201502089

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© 2015 Lambrus et al.

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Figure 8. — De novo centrioles recruit PCM and act as MTOCs. (A) Selected images from a time-lapse series showing de novo centriole formation in cells expressing histone EGFP-Centrin. De novo centrioles duplicate in the second cycle after they are born. Time is indicated in hours relative to the time of IAA washout (time point 0). (B) Quantification of the time of de novo centriole formation relative to the time of cell division. Measurements were made using time-lapse movies of EGFP-Centrin expressing cells from two independent experiments. Line represents the mean of >50 cells. (C) Selected images illustrating the different configurations adopted by de novo centrioles. Cells were costained with Centrin and CEP192 to identify centrioles. (D) Selected images of de novo centrioles in interphase and mitotic cells at 1 and 2 d after IAA washout. The mitotic spindle segregates freestanding de novo centrioles in the first division and pairs of replicated centrioles in the second cell division. Cells were costained with α-tubulin, Centrin, and γ-tubulin or Pericentrin. (E) Selected images of pairs of de novo centrioles in interphase cells at 2 d after IAA washout. Cells were costained with Centrin, CNAP1, and CEP164. Bars: (main) 5 µm; (inset) 1 µm.