Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 6;210(1):957–974. doi: 10.1083/jcb.201410001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — Mitotic progression is impaired in the absence of the Ring/PHD domain. (A) Schematic diagram showing the domain structure of Mio and the sequence of its C-terminal Ring/PHD domain. The predicted cysteines and histidines involved in zinc coordination are labeled in red. (B) Cartoon representation of predicted 3D molecular structure of Mio C-terminal Ring/PHD domain showing the mutated residues in stick form. Zinc ions are shown in ball representation (as white spheres). (C) Mitotic progression scatter plots with NEBD as T = 0 in control (blue), Mio siRNA-treated cells with and without (red) expression of siRNA-resistant versions of wild-type GFP-Mio (green), GFP-Mio^Ring8A (black), and GFP-Mio^ΔPHD (purple) from live cell videos. n = 214 cells from two independent experiments. Statistical significance was determined by an unpaired Student’s t test.