Table 5.
The List of 29 F. graminearum Proteins that Are Expressed during in planta Conditions, Are under Diversifying Selection and Have a Predicted Signal Peptide Using SignalP 4.1 (Petersen et al. 2011)
| ID | Length | [SG]-P-C-[KR]-P Motif | Functional Annotation (MIPS) | Phyre2 Structure Prediction (confidence/coverage) | In planta Barley | In planta Wheat | 16HAI | 40HAI | 64HAI | Pathogen- Associated |
|---|---|---|---|---|---|---|---|---|---|---|
| FGSG_07625 | 325 | — | Probable alpha-L-arabinofuranosidase precursor | Alpha-l-arabinofuranosidase (100%/92%) | Yes | — | Yes | Yes | Yes | — |
| FGSG_10561 | 1480 | — | Related to RF2 protein | — | — | — | — | Yes | Yes | — |
| FGSG_10999 | 231 | — | xylA | Gh11 xylanase (100%/90%) | Yes | — | Yes | — | Yes | — |
| FGSG_04521 | 265 | GPCKP | — | — | — | — | Yes | Yes | — | Yes |
| FGSG_04841 | 273 | GPCKP | — | Endoglucanase (99.3%/54%) | — | — | Yes | — | — | Yes |
| FGSG_05947 | 281 | GKCKP | — | — | — | — | Yes | Yes | — | Yes |
| FGSG_07629 | 278 | GPCKP | — | Endo-1,4-beta-xylanase y (99.5%/53%) | — | — | Yes | Yes | — | Yes |
| FGSG_07684 | 238 | GPCRP | — | Wnt inhibitory factor 1 (99.2%/36%) | — | — | Yes | — | — | — |
| FGSG_12405 | 182 | GPCRP | — | — | — | — | Yes | Yes | — | Yes |
| FGSG_15977 | 295 | GLCKP | — | — | — | Yes | — | — | — | |
| FGSG_02255 | 221 | — | ECP6 (99.1%/32%) | — | — | Yes | — | — | Yes | |
| FGSG_03550 | 186 | — | Related to early nodulin 75 precursor | — | — | — | Yes | — | — | Yes |
| FGSG_04597 | 224 | — | Galactose-binding domain-like (99.9%/72%) | — | — | Yes | — | — | Yes | |
| FGSG_03315 | 411 | — | Related to endopeptidase K | Subtilisin-like (100%/87%) | — | Yes | — | Yes | Yes | — |
| FGSG_13955 | 435 | — | — | — | Yes | Yes | — | Yes | — | |
| FGSG_15136 | 69 | — | — | — | Yes | Yes | Yes | Yes | — | |
| FGSG_15175 | 91 | — | — | — | Yes | — | — | Yes | — | |
| FGSG_03958 | 400 | — | — | Yes | — | — | — | — | — | |
| FGSG_16163 | 513 | — | Homoserine o-acetylytransferase (99.9%/68%) | Yes | — | — | — | — | — | |
| FGSG_01588 | 724 | — | — | — | — | — | Yes | Yes | — | Yes |
| FGSG_12081 | 139 | — | — | — | — | Yes | — | Yes | — | |
| FGSG_03969 | 501 | — | Low density lipoprotein receptor variant (98.6%/81%) | — | — | Yes | — | Yes | — | |
| FGSG_01594 | 163 | — | — | — | — | — | Yes | Yes | — | |
| FGSG_10560 | 251 | — | — | — | — | — | Yes | Yes | — | |
| FGSG_10562 | 286 | — | — | — | — | — | Yes | Yes | — | |
| FGSG_01822 | 268 | — | Ephrin type-a receptor 2 (95.5%/15%) | — | — | — | — | Yes | — | |
| FGSG_08245 | 634 | — | — | — | — | — | — | Yes | — | |
| FGSG_08196 | 260 | — | Related to aspergillopepsin II precursor | Peptidase A4 (100%/75%) | — | — | — | Yes | — | — |
| FGSG_17084 | 373 | — | — | — | — | Yes | Yes | Yes | — |
NOTE.—Two in planta experiments were used, infected barley (Guldener et al. 2006) and infection of wheat as well as specific time points (16 HAI, 40 HAI, and 64HAI) of that experiment (Zhang et al. 2012). Pathogen-associated refers to proteins that have sequence similarity hits specific to fungal pathogens and at the same time predominantly absent from nonpathogenic fungi (Sperschneider et al. 2013).