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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Nature. 2015 May 4;522(7555):226–230. doi: 10.1038/nature14325

Extended Data Figure 7. Small-scale screen for compounds that inhibit epicardial regeneration.

Extended Data Figure 7

Ex vivo ablation and regeneration of tcf21:NTR; tcf21:nucEGFP ventricles over 7 days. Mtz was added for 24 hours to freshly isolated hearts, washed out, and compounds were added after 2 days (Day 0). Hearts were treated with vehicle (n > 10), 10 μM DEAB (n = 5; Sigma-Aldrich), 100 nM LDN193189 (n = 4; Cayman Chemical), 10 μM SU5402 (n = 5; Santa Cruz Biotechnology), 1 μM CyclosporinA (n = 4; Sigma-Aldrich), or 0.1 μg/ml FK506 (n = 5; Sigma-Aldrich), in each case showing base-to-apex recovery (behavior seen in all samples). The dissected hearts were randomly separated into groups for drug treatment. Red dashed lines, epicardial leading edge. White dashed lines, ventricle. Scale bars, 50 μm.