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. Author manuscript; available in PMC: 2015 Dec 11.
Published in final edited form as: Nature. 2015 May 4;522(7555):226–230. doi: 10.1038/nature14325

Extended Data Figure 3. Mosaic NTR expression and patterns of spared epicardial cells after ablation.

Extended Data Figure 3

a, Whole-mounted examples of varied location/pattern of spared epicardial cells in ventricles from tcf21:NTR; tcf21:nucEGFP adult clutchmates 3 days after incubation with 10 mM Mtz. White dashed lines, ventricle. b, Differential expression of the NTR transgene among cardiac chambers. In adult tcf21:NTR; tcf21:nucEGFP hearts, EGFP expression is comparable in epicardial tissue covering the atrium, OFT, and ventricle. By contrast, NTR (red, indicated by mCherry) expression is patchy and/or weak in atrium and OFT compared with ventricular expression. c, Section images of ventricles from tcf21:nucEGFP (left) or tcf21:NTR; tcf21:nucEGFP zebrafish (right) treated with 1 mM Mtz (right) for 3 days, and collected 2 days later. Ventricular epicardium was ablated effectively in these experiments. Scale bars, 50 μm.