Fifty micrograms each of total urothelial proteins obtained from wild-type (WT) or Buff (Buff) mice were separated by SDS–PAGE, and immunoblotted using antibodies against LAMP-1, LAMP-2, UPII, UPIII, Rab27b or actin. Note the significant increase in the relative amounts of both uroplakins and the LAMPs in the urothelium of the Buff mouse when compared with that of WT bladder urothelium.