A.

HUVECs were incubated with IGF-1 (40 ng/ml) for 72 h. The expression levels of miR-29c was descend significantly after IGF-1 treatment compared to control. (B) HUVECs were incubated with different concentrations of IGF-1 for 24 h, the expression levels of miR-29c was detected by qRT-PCR. Control cells were untreated. (C) Immunocytochemical assay shown the IGF1expression levels after treatment. (D) miR-29c directly down-regulates IGF-1 expression. (E) Predict IGF-1 was potential targets for miR-29c and its validation. Luciference reporter assay was performed to detect the effect of miR-29c and anti-sense miR-29c (F) on the luciference intensity controlled by 3’UTR of IGF-1. Mean ± SD (n = 3), *p < 0.05, **p < 0.01.