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. 2014 Mar 5;2(1):181–195. doi: 10.3390/vaccines2010181

Figure 4.

Figure 4

Mycobacteria-specific IFN-γ responses against non plasmid-encoded, but BCG-expressed, antigens after BCG/pDNA co-vaccination. IFN-γ (pg/mL) was measured by ELISA in 72 h culture supernatant of spleen cells cultured with mycobacterial recombinant proteins PstS-3 and Ag85A, BCG culture filtrate (BCG CF) and with recombinant PPE44 (A) or purified ovalbumin (B). Data are presented as the mean ± standard error of the mean and representative of two independent experiments, including six mice per group. * p < 0.05 in comparison with BCG (ID) and BCG/control pDNA (ID) groups.