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. 2013 Dec 27;2(1):36–48. doi: 10.3390/vaccines2010036

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

V-specific IgG (Panel A), IgG1 (Panel B), or IgG2a (Panel C) concentrations measured at 12 weeks after the boost immunization in mice receiving various V vaccine regimens: codon optimized V DNA vaccine alone (V-DNA), V DNA vaccine prime followed by V protein boost formulated with IFA (V-DNA + Prot/IFA), V protein formulated with IFA (V-Prot/IFA), V protein alone (V-Prot), or empty DNA vaccine vector alone (Vector). Antibody titers were measured by ELISA against V protein. Each bar represents the mean IgG concentration in each group of 5 mice with standard error. Statistically significant differences (p < 0.05) are indicated as “*”, “#” or “^” when comparing V-DNA, V-DNA + Prot/IFA, and V-Prot/IFA groups.