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. 2014 Jul 22;2(3):581–600. doi: 10.3390/vaccines2030581

Figure 1.

Figure 1

Expression under control of mPH5 results in degradation of gene products at early time points. Human A375 cells were infected with either MVA-OVA P7.5 or MVA-OVA mPH5 at MOI 10. Cells were harvested at the indicated time points (h p.i., hours post infection) and cell lysates were prepared. (A) Cytosine β-d-arabinofuranoside (AraC), inhibited intermediate and late gene expression; (B) Epoxomicin (Epox), inhibited intermediate and late gene expression and protein degradation by blocking the proteasome.