Table 4.

Objectives of legislation regulating animal research use with references.

Principles	Objectives	Sources
Applying 3Rs	Reducing number of experimental animals keeping scientific value of research	[4,5,36,37,38,39,40]
	Reducing animals’ suffering by refinement such as anaesthesia, humane endpoints, euthanasia
	Replacing animals to non animal tools and use of less sensitive animals
Severity assessment	Recognizing severity of procedures	[4,5,38,40]
Scientific relevance	Reviewing the merit and value of research under review	[4,5,38,39]
	Maximizing benefit
Ethical justification	Ensuring that the benefits of research outweighs the risks (harm-benefit analysis)	[4,5,38,39]
	Giving extra protection to certain species such as non-human primate research
	Preventing duplication of experiments
Expertise of researchers	Well trained personal is involved in experiments	[4,5,38,39,40]
Appropriate design	Methodological rigour of the research is necessary	[4,5,38,39,40]
	Statistical soundness
	Selected species can be scientifically justified