Fig. 1.

Medikhan procedure. A, Medikhan centrifuge. B, The fat (80 ml) was centrifuged at 4000 rpm for 8 minutes and placed in 60-ml syringes; extraction of syringes at the end of centrifugation. C, Collagenase, 1 ml of collagenase and 24 ml of saline solution and 25 ml of condensated fat. D, The syringe with condensated fat was enzymatically digested in the TP102 syringe Celltibator, containing 1 ml of collagenase and 24 ml of saline solution and was aseptically inserted in the incubator for the isolation of SVF by a slow centrifugation for 30 minutes at 200 rcf.