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Effect of calcium ions (Ca2+) on the onset of mineralization in human dental pulp cells at day 3 and 5. (A) Representative micrographs prior to staining. (B) Images of Alizarin Red S staining. Obvious calcium deposition was observed in the groups treated with 5.4 and 9.0 mM Ca2+ (Original magnification, ×100). (C) General observation of culture wells stained with Alizarin Red S.
