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. 2015 May 22;36(1):195–203. doi: 10.3892/ijmm.2015.2219

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Combined treatment with insulin-like growth factor-I (IGF-I) and ethyl pyruvate (EP) increases hypoxic-ischemic (HI) injury-induced neurogenesis. (A) High magnification view of the square in the hippocampus. Double immunostaining to identify the newborn neurons (panel a, merge) with doublecortin (DCX) immunostaining (panel b, red) and bromodeoxyuridine (BrdU) immunostaining (panel c, green) 72 h post-HI injury. Nuclei are stained with DAPI (panel d, blue). (B) Quantification of the number of newborn neurons (DCX⁺BrdU⁺). ^*P<0.05, ^**P<0.01 as indicated (n=6).