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. 2015 May 22;36(1):195–203. doi: 10.3892/ijmm.2015.2219

Figure 7.

Figure 7

Combined treatment with insulin-like growth factor-I (IGF-I) and ethyl pyruvate (EP) promotes the maturation of newborn neurons. (A) Matured neurons born in the hippocampus. Double immunostaining to identify the matured neurons (panel a, merge) with neuronal nuclei antigen (NeuN) (panel b, red) and bro-modeoxyuridine (BrdU) (panel c, green) at 4 weeks post-hypoxic-ischemic (HI) injury. Nuclei are stained with DAPI (blue; panel d). (B) High magnification view of the square in the hippocampus to show the mature neurons (panel a, merge) with NeuN antibody (panel b) and antibody against BrdU (panel c) 4 weeks post-HI injury. Nuclei are stained with DAPI (blue;panel d). (C) Quantification of the number of newborn neurons (NeuN+BrdU+). *P<0.05, **P<0.01 as indicated (n=6).