Figure 5.

RT-qPCR of survivin mRNA expression following transfection with lentivirus (LV) carrying survivin in the transfected ccells and subsequent generation cells (passage 3). The expression of survivin following transfection was significantly higher than that in the control groups (p<0.01). No significant difference in the expression levels was observed between the transfected cells and subsequent generation cells, as well as between the negative and the blank control groups (p>0.05). Positive group, transfection with lentivirus carrying survivin; negative group, transfection with empty lentivirus; blank group, transfection with an equal amount of DMEM/F12 medium.