Figure 2.

(A) Quantification (% of calcification) of calcium deposition in the aortae of 22-week-old Enpp1^−/− mice. A standardised region of calcium deposition (400 slices from the subclavian artery) was selected and revealed no significant differences between the placebo (vehicle; saline)- and etidronate-treated groups. (B) Three-dimensional volumetric reconstructions of aortae from 22-week-old wild-type (WT) placebo-treated, WT etidronate-treated, Enpp1^−/− placebo-treated, and Enpp1^−/− etidronate-treated mice. Calcification is indicated by brown colouring. Bar represents 0.5 mm.