Figure 2.

ACC/CaIP₆ nanoparticle siRNA-binding efficiency and cytotoxicity in MCF-7 cells.

Notes: (A) Gel electrophoresis was used to assess ACC/CaIP₆ nanoparticles and siRNA binding at different mass ratios. (B) Cytotoxicity was measured by the percentage of viable MCF-7 cells present after ACC/CaIP₆ treatment, using the MTT assay 48 hours post–transfection. These percentages were calculated in relation to those of untreated controls. The data show the mean ± standard deviation of three independent experiments (^*P<0.05 by one-way analysis of variance).

Abbreviations: ACC/CaIP₆, amorphous calcium carbonate hybrid nanospheres functionalized with a Ca(II)-inositol hexakisphosphate compound; siNC, control small interfering RNA; lipo, Lipofectamine 2000; siRNA, small interfering RNA.