Figure 6.

Tumor growth in vivo after intratumoral injections of ACC/CaIP₆/siAKT1 nanoparticles.

Notes: (A) Representative tumors before and after injection. (B) ACC/CaIP₆/siAKT1 (20 µg siAKT1/injection, 50:1 mass ratio) was locally injected into the tumors (100 mm³ in volume at 12 days). Tumor diameter was measured using calipers and tumor volume was calculated using the following formula: volume = W² × L/2, where W is the width of the tumor and L is the tumor length. Results are presented as the mean ± standard deviation (n=7 per group; ^***P<0.001 versus other groups). (C) Mean tumor weight (± standard deviation) after mice were sacrificed (n=7 per group; ^**P<0.01 versus other groups).

Abbreviations: ACC, amorphous calcium carbonate; ACC/CaIP₆, amorphous calcium carbonate hybrid nanospheres functionalized with a Ca(II)-inositol hexakisphosphate compound; siAKT1, small interfering AKT1.