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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Science. 2015 Apr 30;348(6239):1160–1163. doi: 10.1126/science.aaa1356

Fig. 1. WRN-deficient MSCs exhibit phenotypes associated with premature cellular senescence.

Fig. 1

(A) Morphology and immunofluorescence analyses of pluripotency markers in ESCs. Scale bar, 100 μm and 10 μm, respectively. (B) Western blot analysis of WRN expression in ESCs using anti-WRN N-terminal (ab200) and C-terminal (SC-5629) antibodies. (C) Growth curve analyzing the accumulative population doubling of MSCs. (D) Senescence-associated (SA)-β-gal staining in passage 1 (P1) and P5 MSCs. Scale bar, 50μm. (E) Quantitative RT-PCR analysis of the indicated genes in P1 and P5 MSCs. Transcript levels were normalized to MSCs-WRN+/+ group. Genes with greater mean value are color coded towards red. (F) Photon flux from muscle of NOD-SCID mouse transplanted with MSCs-WRN+/+ (left) and MSCs-WRN−/− (right) expressing luciferase. All data are represented as mean + SEM. *P<0.05, **P<0.01, ***P<0.001 by t test; n=3.