Table 3.
Plasmids used in this study
| Plasmid | Description | Reference/origin |
|---|---|---|
| pNW33n | E. coli-Bacillus shuttle vector, cloning vector, CmR | BGSC |
| pWUR732 | ldhL-KO vector for ET138: pNW33n + ldhL-flanks | This study |
| pWUR733 | ldhL-KO vector for DSM 4216: pNW33n + ldhL-flanks | This study |
| pWUR734 | pNW33n + B. coagulans Ppta-lacZ | [14]; this study |
| pWUR735 | sigF-KO vector for ET138: pWUR734 + ET 138 sigF-flanks | This study |
| pWUR736 | ldhL-restoration vector for ET138: pNW33n + ldhL-gene from ET 138 under its native promoter (525 bp us of ldhL) | This study |
| pWUR737 | pdhA-KO vector for ET138: pWUR734 + ET 138 pdhA -flanks | This study |
Cm R chloramphenicol resistance gene, KO knockout, BGSC Bacillus Genetic Stock Centre, USA, us upstream, bp base pairs.