Table 4.
Primers used in this study
| BG nr | Sequence 5′–3′ | Purpose |
|---|---|---|
| 3464 | AACTCTCCGTCGCTATTGTAACCA | Check plasmid presence |
| 3465 | TATGCGTGCAACGGAAGTGAC | Check plasmid presence |
| 3633 | GCCGTCGACCATTTGCAGTAGGTCTCGATC | ldhL-us-Fw |
| 3636 | GCCGAATTCTAGGTCACCAAAGACGAAATTG | ldhL-ds-Rv |
| 3637 | GCTCCCTTTGTATGGTCGTTTACATAATAAGAAACTCCTTTCGTCATTTC | ldhL-us-Rv |
| 3638 | GAAATGACGAAAGGAGTTTCTTATTATGTAAACGACCATACAAAGGGAGC | ldhL-ds-Fw |
| 3664 | AGGGCTCGCCTTTGGGAAG | Int. check, in plasmid |
| 3663 | ATCGCGTGAAATGTTCTAATGG | Int. check ldhL on chr.-Fw |
| 3669 | AACCGATGCCGTTGATTAAAG | Int. check ldhL on chr.-Rv |
| 3887 | GCCGAGCTCTTGCCGGAATTCTTTCAC | pta-lacZ-Fw |
| 3888 | GCCTCATGACTATTTTTCAATTACCTGCAAAATTTTC | pta-lacZ-Rv |
| 3971 | GCCGAATTCAGCTAATCTTGTTGACGGTTTTC | sigF-ds-Fw |
| 3972 | GTAACTAAGGAGTCGTGCCTTAACGATTCATGTGCTTTTTTTTG | sigF-ds-Rv |
| 3973 | CAAAAAAAAGCACATGAATCGTTAAGGCACGACTCCTTAGTTAC | sigF-us-Fw |
| 3974 | GCCGTCGACCTCTGATTTAGAAGATGGAGGTTTT | sigF-us-Rv |
| 3990 | CGCCTATTCTTTTCGCTAAAATCGG | Int. check sigF on chr.-Fw |
| 3991 | ATAAGCTGCAGAGGGATATACAC | Int. check sigF on chr.-Rv |
| 4534 | GCCTCTAGAATTGGTCATTTGATTAGA | ET 138 ldhL + prom.-Fw |
| 4535 | GCCAAGCTTTTAAGAAAGTACTTTATT | ET 138 ldhL + prom.-Rv |
| 4522 | GCCGAATTCGAGGTACATAGCCCGGAATC | pdhA-us-Fw |
| 4523 | GTCATTTGCGGCATGGCTTACATTCGTGTCACCTCTTCCTTTC | pdhA-us-Rv |
| 4524 | GAAAGGAAGAGGTGACACGAATGTAAGCCATGCCGCAAATGAC | pdhA-ds-Fw |
| 4525 | GCCGTCGACCATCCTCATAACGGCCATCC | pdhA-ds-Rv |
| 4563 | GTTTCACATACCATTTAACGATTT | Int. check pdhc on chr.-Rv |
| 4564 | GTCAATAGGTGCAAATGGATTTTC | Int. check pdhc on chr.-Fw |
us upstream flanking region, ds downstream flanking region, Fw forward primer, Rv reverse primer, Int. integration, chr. chromosome, prom. promoter.