Table 1.
Primer sequences for site-directed mutagenesis of NeuroD4
| Mutation | Sense primer 5′ to 3′ | Anti-sense primer 5′ to 3′ |
|---|---|---|
| Xath_S197A | CATACTTATAATTACCAGGCCCCAGGACTACCCAG | CTGGGTAGTCCTGGGGCCTGGTAATTATAAGTATG |
| Xath_S202A | GGCCCCAGGACTACCCGCTCCTCCTTATGGTAAC | GTTACCATAAGGAGGAGCGGGTAGTCCTGGGGCC |
| Xath_T237A | CATACACTTAACTGTACCGCTCCACCATATGAAGGAG | CTCCTTCATATGGTGGAGCGGTACAGTTAAGTGTATG |
| Xath_T245A | TCCACCATATGAAGGAGCTCTAGCACCTCCACTCA | TGAGTGGAGGTGCTAGAGCTCCTTCATATGGTGGA |
| Xath_S261A | GGTAATTTTTCTTTGAAGCAAGATAGTGCACCCGATATGGATAAAT | ATTTATCCATATCGGGTGCACTATCTTGCTTCAAAGAAAAATTACC |
| Xath_S272A | AATCATATGCATTCAGGGCCCCCTATCCAGCTCTT | AAGAGCTGGATAGGGGGCCCTGAATGCATATGATT |