. Author manuscript; available in PMC: 2015 Jul 7.

Published in final edited form as: J Nucl Med. 2013 Dec 23;55(2):321–328. doi: 10.2967/jnumed.113.121053

Table 3.

Summary of the reaction conditions for the radiosynthesis of [¹⁸F]FLT on EWOD chip based on Method 2.

Process	Temp. (°C)	Reaction time (min)	Reagent	Conc.(mM)	Droplet volume (μL)	Fluorination efficiency (%)^†	Radioactivity remained on chip (%)^*	Crude Radiochemical yield (%)^†
(1) Load [¹⁸F]TBAF	RT		TBAHCO₃	45	6	n/a		n/a
(2) MeCN azeotropic distillation	105	2	MeCN	n/a	9	n/a		n/a
(3) Fluorination	120	3	FLT precursor	90	2	91±4 (n=10)	94 (n=4)	n/a
(4) Hydrolysis	95	1.5	HCl	75	7	n/a	85 (n=4)	82±10 (n=11)

To determine radioactivity remaining on chip, the chip was removed after each step and the radioactivity on the entire chip was measured using a dose calibrator for the radioactivity measurement.

^†

Fluorination efficiencies and yields are reported with standard deviations based on the number of experiments, n.