Table A2.
CMV shedding prevalence according to risk group, categorized by whether single specimens (SS) or multiple specimens (MS) were used and whether they were collected at a single time point (ST) or multiple time points (MT)a
| Country | Demographics/Sampling Method | Testing Method | Specimen Type(s) | Category | Sample size | Shedding % |
|---|---|---|---|---|---|---|
| Category I. Congenitally infected children | ||||||
| Sweden [59] | 35 congenitally infected infants, ages 3 months-4 years | Culture | Urine | SS, ST | 149 | 84 |
| US-Alabama [75] | 38 congenitally infected infants, ages 1–4 years | Culture | Urine | SS, ST | 69 | 87 |
| Category II. Healthy children enrolled in day care centers | ||||||
| England [98] | 117 children in day nurseries over a 1-year period, 2–4 specimens per year | Culture | Urine | SS, MT | 117 | 27 |
| France [65] | 93 children < 1 year old in day care centers | Culture | Urine | SS, ST | 379 | 24 |
| SS, MT | 93 | 36 | ||||
| Italy [69] | 253 children in day care centers | Culture | Oral secretions | SS, ST | 253 | 13 |
| Japan [64] | 54 children in day care centers | PCR | Oral secretions | SS, ST | 54 | 22 |
| Mexico [77] | 152 children at day care centers, sampled at 3 visits | Culture | Oral secretions | SS, ST | 127 | 7 |
| SS, ST | NS | 5 | ||||
| SS, ST | NS | 7 | ||||
| SS, MT | 152 | 11 | ||||
| Sweden [122] | 60 children in day care centers followed 12 weeks | Culture | Urine | SS, MT | 60 | 27 |
| Sweden [68] | 18 children in day care centers, ages 2–3.5 years, mean follow-up of 35 weeks | Culture | Urine | SS, ST | 221 | 55 |
| US-Alabama [72] | Children in day care centers | Culture | Urine, oral secretions | MS, ST | 243 | 32 |
| US-Alabama [97] | 188 children in day care centers | Culture | Urine, oral secretions | MS, ST | 188 | 41 |
| US-Alabama [14] | Children in day care centers | Culture | Urine, oral secretions | MS, ST | 71 | 51 |
| US-Alabama [56] | 103 children in day care centers, 1–3 visits each | Culture | Urine, oral secretions | MS, MT | 103 | 57 |
| US-California [63] | 100 children in day care centers | Culture | Urine, oral secretions | MS, ST | 90 | 22 |
| US-Iowa [66] | 48 children in day care centers | Culture | Urine, oral secretions | MS, ST | 80 | 21 |
| US-Iowa [67] | 79 children in day care centers followed for 2.5 years | Culture | Urine, oral secretions | MS, MT | 79 | 35 |
| US-Iowa [13] | 219 children in day care centers at entry | Culture | Urine, oral secretions | MS, ST | 219 | 15 |
| 219 children in day care centers followed for 2.5–4.5 years | Urine, oral secretions | MS, MT | 219 | 28 | ||
| US-Virginia [11] | 66 children in day care centers cultured 3 times | Culture | Urine | SS, MT | 66 | 24 |
| US-Virginia [123] | Children in day care centers at 2 time points | Culture | Urine, oral secretions | MS, ST | 118 | 44 |
| Category III. Healthy children not enrolled in day care centers | ||||||
| England [62] | Infants without congenital CMV | Culture | Urine | SS, ST | 983 | 12 |
| The Gambia [61] | 178 infants | Culture | Urine, oral secretions | MS, ST | 178 | 53 |
| MS, MT | 150 | 62 | ||||
| Japan [64] | 61 children not in day care centers | PCR | Oral secretions | SS, ST | 61 | 7 |
| Japan [88] | Infants and children ages 1 month-2 years | Culture | Oral secretions | SS, ST | 290 | 25 |
| Mauritius [58] | 121 healthy children | Culture* | Urine | SS, ST | 121 | 2 |
| Sweden [59] | 50 children without congenital CMV, ages 3 months-4 years | Culture | Urine | SS, ST | 225 | 31 |
| US-Alabama [57] | 87 healthy infants ages 9–15 months | Culture | Oral secretions | SS, ST | 87 | 7 |
| US-Alabama [56] | 25 children in home care | Culture | Urine, oral secretions | MS, ST | 25 | 8 |
| US-Iowa [60] | 106 children in child care homes | Culture | Urine | SS, ST | 106 | 8 |
| US-New York [32] | 200 healthy children | Culture | Urine | SS, ST | 200 | 1 |
| US-New York [30] | 38 healthy migrant children | Culture | Urine | SS, ST | 38 | 18 |
| Category IV. Children with medical conditions | ||||||
| Denmark [89] | 262 hospitalized infants, not congenitally infected | Culture | Urine | SS, ST | 262 | 4 |
| England [99] | Hospitalized children | Culture | Urine, oral secretions | MS, ST | 1395 | 3 |
| England [19] | 309 hospitalized patients, ages 2 months-14 years | Culture | Urine, oral secretions | MS, ST | 309 | 5 |
| Finland [31] | 356 hospitalized and outpatient children > 2 months of age | Culture | Urine | SS, ST | 356 | 26 |
| Mauritius [58] | 30 deaf children | Culture | Urine | SS, ST | 30 | 50 |
| 91 mentally retarded children | 91 | 18 | ||||
| Sweden [90] | 661 hospitalized infants | Culture | Urine | SS, ST | 661 | 9 |
| US-California [63] | 63 children in infant development centers | Culture | Urine, oral secretions | MS, ST | 50 | 22 |
| US-California [114] | 93 infants in the intensive care unit, cultured weekly until discharge | Culture | Urine | SS, MT | 93 | 14 |
| US-New York [32] | 100 hospitalized children | Culture | Urine | SS, ST | 100 | 1 |
| US-Texas [124] | 314 hospitalized children in chronic care | Culture | Oral secretions, urine, genital secretions | MS, ST | 314 | 9 |
| US-Virginia [11] | 2729 hospitalized children from newborns up to 18 years old | Culture | Urine | SS, ST | 2729 | 5 |
| V. Adults without CMV risk factors | ||||||
| Egypt [87] | 50 women with recurrent abortions | PCR | Serum | SS, ST | 50 | 12 |
| England [19] | 402 hospitalized patients, ages 15–60+ years old | Culture | Urine, oral secretions | MS, ST | 402 | 0 |
| France [91] | 231 semen donors | Culture | Semen | SS, ST | 635 | 1 |
| SS, MT | 231 | 1 | ||||
| PCR | SS, ST | 551 | 4 | |||
| SS, MT | 197 | 3 | ||||
| France [93] | 97 semen donors | Culture | Semen | SS, ST | 178 | 3 |
| SS, MT | 97 | 2 | ||||
| PCR | SS, ST | 178 | 6 | |||
| SS, MT | 97 | 5 | ||||
| The Gambia [61] | 178 post-partum women, average prevalence in ≥1 of 3 specimen types over 6 cross-sectional visits | Urine, oral secretions, breast milk | MS, ST | 178 | 12 | |
| Greece [92] | 113 men at an infertility clinic | Nested PCR | Semen | SS, ST | 113 | 7 |
| Italy [82] | 66 women receiving routine gynecological care | PCR | Genital secretions | SS, ST | 66 | 73 |
| Italy [85] | 123 patients with fever of unknown origin | PCR | Serum | SS, ST | 123 | 9 |
| Japan [80] | 953 blood donors | PCR | Blood | SS, ST | 953 | 3 |
| Japan [81] | 993 healthy pregnant women, CMV serostatus undetermined, first trimester | PCR | Genital secretions | SS, ST | 993 | 8 |
| Taiwan [83] | 29 post-partum women, CMV serostatus undetermined | PCR | Serum | SS, ST | 29 | 10 |
| Taiwan [71] | 350 pregnant women at 2 time points in the 2nd trimester, all CMV seropositive | PCR | Urine | SS, MT | 350 | 11 |
| Genital secretions | SS, MT | 220 | 30 | |||
| Taiwan [22] | 105 non-pregnant women from an infertility clinic | PCR | Genital secretions | SS, ST | 105 | 8 |
| Taiwan [95] | Men and women attending infertility clinic | DNA hybridization | Genital secretions | SS, ST | 246 | 34 |
| Semen | SS, ST | 248 | 34 | |||
| Turkey [76] | 135 pregnant women, CMV serostatus undetermined, first trimester | PCR | Genital secretions | SS, ST | 135 | 2 |
| US-Alabama [86] | 113 healthy, post-partum women, all CMV seropositive, mean of 4.9 specimens per woman | PCR | Urine, blood | MS, MT | 113 | 14 |
| Urine | SS, MT | 113 | 8 | |||
| Blood | SS, MT | 113 | 6 | |||
| US-Alabama [73] | 81 post-partum women, all CMV seropositive, over an average of 4 visits, with uninfected infants | Culture | Urine, Oral secretions, genital secretions | MS, MT | 81 | 23 |
| US-Massachusetts [94] | 241 male infertility clinic attendees | PCR | Semen | SS, ST | 241 | 9 |
| US-New York [70] | 181 HIV-negative, CMV-seropositive urban, minority women | Culture | Genital secretions | SS, ST | 181 | 4 |
| US-Ohio [121] | 710 CMV seropositive adolescent pregnant women cultured at each antenatal visit | Culture | Urine | SS, MT | 710 | 17 |
| US-Texas [124] | 43 nurses in chronic care | Culture | Oral secretions, urine, genital secretions | MS, ST | 43 | 2 |
| 76 therapists in chronic care | 76 | 3 | ||||
| 69 neonatal nurses | 69 | 3 | ||||
| US-Virginia [12] | 239 CMV seropositive day care workers, average of 3.4 culture sets per woman over two years | Culture | Urine, oral secretions | MS, ST | 239 | 7 |
| MS, MT | 239 | 11 | ||||
| US-Washington [125] | 120 critically ill immunocompetent patients, all CMV seropositive, 1954 samples, 3 samples collected per week, median of 11 samples per patient | PCR | Plasma | SS, MT | 120 | 33 |
| Category VI. Adults with CMV risk factors | ||||||
| Italy [82] | 142 women with abnormal cytology findings | PCR | Genital secretions | SS, ST | 142 | 63 |
| Malawi [126] | 19 KS patients and 58 first-degree family members | PCR | Urine, oral secretions | MS, ST | 77 | 53 |
| US-Alabama [73] | 142 post-partum women, all CMV seropositive, over an average of 4 visits, with infected infants | Culture | Urine, oral secretions, genital secretions | MS, MT | 142 | 56 |
| US-Alabama [79] | 52 women who were attending an STD clinic | PCR | Genital secretions | SS, ST | 52 | 33 |
| US-California [21] | 206 CMV seropositive homosexual men, HIV status unknown, followed for a mean of 14.3 months | Culture | Urine | SS, MT | 206 | 32 |
| US-New York [100] | 30 healthy homosexual men | Culture | Urine, semen | MS, ST | 30 | 37 |
| US-Washington [39] | 191 CMV seropositive women attending an STD clinic, most were symptomatic, mean of 2.7 follow-up visits | Culture | Genital secretions | SS, ST | 163 | 38 |
| SS, MT | 191 | 49 | ||||
| US-Washington [20] | 951 CMV seropositive women presenting with problems at an STD clinic | Culture | Urine, genital secretions | MS, ST | 951 | 17 |