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. Author manuscript; available in PMC: 2015 Jul 7.
Published in final edited form as: Nat Neurosci. 2014 Apr 22;17(6):884–889. doi: 10.1038/nn.3709

Figure 3.

Figure 3

Monitoring simulated hyperpolarizations and subthreshold potentials in voltage-clamped neurons. (a) ASAP1 can detect subthreshold potential and hyperpolarization waveforms in cultured hippocampal neurons. Subthreshold depolarizations and hyperpolarizations have peak amplitudes of 5, 10, 15, and 20 mV, and peak full width at half maximum of 17 ms (depolarizations) and 38 ms (hyperpolarizations). (b) Quantification of the fluorescence responses to subthreshold depolarizations (top) and hyperpolarizations (bottom). Asterisks identify statistically significant differences from pairwise two-tailed t-tests adjusted for multiple comparisons using the Holm-Bonferroni method (p = 0.006, −5 mV waveform; p = 0.0005, 0.008, 0.007 and 0.003 for the −5, −10, −15 and −20mV waveforms, respectively). Multiple comparisons adjustments were performed separately for depolarizations and hyperpolarizations. n = 6 (ASAP1) and 4 (ArcLight Q239) neurons from the same litter. Error bars, SEM. (c) ASAP1’s faster kinetics allow improved resolution of a 100-Hz, three-AP waveform sequence in cultured hippocampal neurons (ASAP1, n = 8 neurons; ArcLight Q239, n = 6 neurons; all cells from same litter). Command voltage spike FWHM is 1.8 ms. Additional examples are in Supplementary Fig. 7.