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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Protoc. 2015 Jun 4;10(7):974–984. doi: 10.1038/nprot.2015.058

Figure 3.

Figure 3

Anticipated results from Step 27 showing the DNase I random fragmentation of amplified ds-cDNA on an agarose gel. Serial dilutions of DNase I (Epicentre) were used for fragmentation at 37°C for 5 min, and different levels of fragmentation were observed as shifted DNA smears. Lane M, 1 kb DNA ladder from New England Biolabs. Lane 1, 1 U of DNase per μg of DNA; lane 2, 0.1 U of DNase per μg of DNA; lane 3, 0.01 U of DNase per μg of DNA; lane 4, 0.001 U of DNase per μg of DNA; lane 5, 0.0001 U of DNase per μg of DNA; lane 6, no DNase control.