| Components | Amount (μL) | Final Concentrations |
|---|---|---|
| GTP (100 mM) | 0.04 | 4 nmol |
| McrBC (10 U μl−1) | 0.1 | 1 U |
| Dpnl (20 U μl−1) | 0.1 | 2 U |
| Total Volume | 0.24 |
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