| Components | Amount (μl) | Final Concetrations |
|---|---|---|
| dATP (100 mM) | 47.6 | 25 mM |
| dCTP (100 mM) | 47.6 | 25 mM |
| dGTP (100 mM) | 47.6 | 25 mM |
| dTTP (100 mM) | 28.5 | 15 mM |
| aa-dUTP (1 mg) | 1 vial | 10 mM |
| KPO4 (0.1 M, pH 8.5) | 19.1 | – |
| Total Volume | 190.4 |
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