| Components | Amount (μl) | Final Concentrations |
|---|---|---|
| Nuclease-free ddH2O | 2.81 | – |
| Φ29 reaction buffer (10x) | 0.45 | 1x |
| DTT (100 mM) | 0.2 | 20 nmol |
| dNTPs/aa-dUTP stock from Step 13 (25 mM) | 0.5 | 12.5 nmol each |
| RNA random hexamers 6R5S (30 pmol μl−1) | 0.5 | 15 pmol |
| GTP (100 mM) | 0.04 | 4 nmol |
| Total Volume | 4.5 |
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