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Deposition of cartilage-specific ECM by hMSCs cultured in the cartilage phase of the biphasic scaffold compared to the silk scaffold after 21 days in chondrogenic medium, as assessed by (A–D) Alcian blue staining for proteoglycans (blue = sulfated GAGs, red = cell nuclei), and (E–H) collagen type II immunofluorescence (red = collagen type II, green = cell nuclei). Asterisks indicate the silk matrix.
